Efficient expression and characterization of hepatitis B virus preS2 antigen in Escherichia coli

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Title
Efficient expression and characterization of hepatitis B virus preS2 antigen in Escherichia coli
Author(s)
Young Jun Kang; Young Shin Kim; Hyang Suk Hur; Hee Sun Kim; Hyo Jeong Hong
Bibliographic Citation
Biotechnology Letters, vol. 21, pp. 375-380
Publication Year
1999
Abstract
The complete (encoding 55 amino acids, aa) or partial (encoding aa 1-26) preS2 region gene of hepatitis B virus (HBV) was fused to the 3'-end of glutathion-S-transferase (GST) gene and expressed under the control of the inducible tac promoter in Escherichia coli at 37°C. The fusion protein with the complete preS2 region was moderately expressed (8%) while the protein with the N-terminal 26 aa was expressed at a higher level, yielding about 20% of the total cellular proteins. The GST-preS2 (aa 1-26) protein, which contains the immunodominant epitope, was produced form the soluble protein fraction of the recombinant bacteria and purified by affinity chromatography using glutathione-agarose column. The purified preS2 fusion protein showed the antigenicity of preS2, as assessed by indirect and competitive ELISAs.
Keyword
Escherichia coliExpressionhepatitis B viruspreS2 antigen
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005487105048
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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