Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive

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Title
Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive
Author(s)
Dong Hyun Kim; Byung Chul Oh; Won Chan Choi; Jung Kee Lee; Tae Kwang Oh
Bibliographic Citation
Biotechnology Letters, vol. 21, pp. 925-927
Publication Year
1999
Abstract
Phytases from Bacillus amyloliquefaciens DS11 and Aspergillus ficuum for feed enzyme were compared on the basis of phosphate inhibition and thermal stabilities. The apparent half-life of the former enzyme at 80 °C was 42 min. The activity of B. amyloliquefaciens phytase was retained up to 5 mM phosphate while 3 mM phosphate inhibited 50% of the original activity in case of A. ficuum phytase. Addition of 5 mM CaCl2 significantly broadened the active pH range and also increased the pH stability of DS11 phytase.
Keyword
calciumpH stabilityphosphate inhibitionphytasethermal stability
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005602717835
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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