Extracellular secretion of levansucrase from Zymomonas mobilis in Escherichia coli

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Title
Extracellular secretion of levansucrase from Zymomonas mobilis in Escherichia coli
Author(s)
K H Jang; Jeong Woo Seo; Ki Bang Song; Chul Ho Kim; Sang Ki Rhee
Bibliographic Citation
Bioprocess Engineering, vol. 21, no. 5, pp. 453-458
Publication Year
1999
Abstract
Secretion of levansucrase from Zymomonas mobilis in Escherichia coli by glycine supplement was investigated. A significant amount of levansucrase (about 25% of total activity) was found in intact whole-cells. Cell fractionation experiments showed that levansucrase was found both in the periplasmic space and in the cytoplasmic fraction of E. coli. None or only trace amounts of levansucrase was detected in the extracellular culture broth at 24 h of cultivation and it accrued with the increasing concentration of glycine in the culture medium and duration of the culture period. Optimal glycine concentration for the maximum secretion of levansucrase was in the range of 0.8-1%, in which approximately 20-50% of levansucrase was released into the extracellular fraction at 24 h of cultivation, although glycine retarded the bacterial growth.
ISSN
0178-515X
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s004490050701
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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