High frequency shoot induction and plant regeneration from cotyledonary hypocotyl explants of Cucumber(Cucumis sativus L.) seedlings

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Title
High frequency shoot induction and plant regeneration from cotyledonary hypocotyl explants of Cucumber(Cucumis sativus L.) seedlings
Author(s)
Jae Whune Kim; Su Kyung Han; Suk Yoon Kwon; Heang Soon Lee; Yong Pyo Lim; Jang Ryol Liu; Sang Soo Kwak
Bibliographic Citation
Journal of Plant Physiology, vol. 157, no. 1, pp. 136-139
Publication Year
2000
Abstract
A method of high frequency shoot induction was established using excised hypocotyl segments bearing cotyledons (referred to as cotyledonary hypocotyl explants) of cucumber (Cucumis sativus L.) seedlings. Adventitious shoots were obtained from the upper excised hypocotyls of cucumber seedlings incubated on Murashige and Skoog (MS) medium containing 2.0 mg/L zeatin. The effects of cotyledon size and hypocotyl length of seedlings on shoot organogenesis were also examined. A higher frequency (80-90 %) of adventitious shoots was obtained from explants of 3- and 5-day-old seedlings with either 1 cotyledon or 2 half-cotyledons, and a short hypocotyl length (2 mm). However, explants with long hypocotyls and 2 cotyledons produced fewer shoots. Adventitious shoots from cotyledonary hypocotyl explants were removed and rooted in MS medium containing 1.0 mg/L IAA. The rooted plantlets developed into normal plants after acclimatization to ambient humidity levels.
Keyword
cucumis sativushypocotyl segmentplant regenerationshoot induction
ISSN
0176-1617
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0176-1617(00)80147-9
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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