Screening of microorganisms producing esterase for the production of (R)-β-acetylmercaptoisobutyric acid from methyl (R,S)-β-acetylmercaptoisobutyrate

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Title
Screening of microorganisms producing esterase for the production of (R)-β-acetylmercaptoisobutyric acid from methyl (R,S)-β-acetylmercaptoisobutyrate
Author(s)
Boyapati Gokul; Je Hyuk Lee; Ki Bang Song; T Panda; Sang Ki Rhee; Chul Ho Kim
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 5, no. 1, pp. 57-60
Publication Year
2000
Abstract
(R)-β-acetylmercaptoisobutyric acid (RAM), a chiral compound, is an important intermediate for the chemical synthesis of various antihypertensive and congestive heart failure drugs. Microorganisms capable of converting (R,S)-β-acetylmercaptoisobutyric acid ((R,S)-ester) to RAM were screened from soil microorganisms. A strain of Pseudomonas sp. 1001 screened from a soil sample was selected to be the best. Cells showed an activity of 540 U/mL from culture broth and the enzyme was thermostable up to 70°C. This strain could produce RAM asymmetrically from (R,S)-ester.
Keyword
(R)-β-acetylmercaptoisobutyric acidPseudomonas sp.esteraseenzymatic resolution
ISSN
1226-8372
Publisher
South Korea
DOI
http://dx.doi.org/10.1007/BF02932355
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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