A simplified procedure for the purification of human N-acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues
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- A simplified procedure for the purification of human N-acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues
- Eun Young Song; Kyoung A Kim; Yung Dai Kim; Dur Han Kwon; Hong Soo Lee; Cheorl Ho Kim; Tae Wha Chung; Si Myung Byun
- Bibliographic Citation
- Biotechnology Letters, vol. 22, no. 3, pp. 201-204
- Publication Year
- Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N- acetylglucosaminyltransferase-III. With the new procedure, human N- acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold.
- hepatocellular carcinomaimmunoaffinity columnmonoclonal antibodyN-acetylglucosaminyltransferase
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- 1. Journal Articles > Journal Articles
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