Antiphotooxidative activity of protoberberines derived from coptis japonica makino in the chlorophyll-sensitized photooxidation of oil

Cited 36 time in scopus
Metadata Downloads
Title
Antiphotooxidative activity of protoberberines derived from coptis japonica makino in the chlorophyll-sensitized photooxidation of oil
Author(s)
Jin Pyo Kim; Mun Yhung Jung; Jong Pyung Kim; Sung Yeol Kim
Bibliographic Citation
Journal of Agricultural and Food Chemistry, vol. 48, no. 4, pp. 1058-1063
Publication Year
2000
Abstract
Antiphotooxidative components were isolated from the methanolic extract of Coptis japonica Makino by liquid-liquid partitioning fractionation, subsequent column chromatography on Sephadex LH20 and silica gel, and preparative silica gel TLC. The isolated compounds were identified as coptisine, jatrorrizhine, berberine, and magnoflorine by a combination of spectroscopic studies using UV-visible, IR, mass-spectrometry, and NMR. Coptisine, jatrorrizhine, and berberine isolated from Coptis japonica Makino showed strong antiphotooxidative activity in the chlorophyll-sensitized photooxidation of linoleic acid. However, these compounds did not show either inhibitory activity against lipid peroxidation in rat liver microsomes nor DPPH radical scavenging activity, indicating that their antiphotooxidative activity was not due to the radical chain reaction breaking ability but due to singlet oxygen quenching activity. Commercially available authentic protoberberines (berberine chloride and palmatine chloride) also showed strong antioxidative activity in the chlorophyllsensitized photooxidation of linoleic acid. The antiphotooxidative activities of the berberine chloride and palmatine chloride were significantly higher than that of ascorbyl palmitate in the chlorophyllsensitized photooxidation of linoleic acid. These results clearly showed for the first time the antiphotooxidative properties of protoberberines in chlorophyll-sensitized photooxidation of oil.
Keyword
Coptis japonica Makinophotooxidationberberinecoptisinejatrorrizhinemagnoflorinechlorophyllssinglet oxygen
ISSN
0021-8561
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/jf9909297
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.