Local structural elements in the mostly unstructured transcriptional activation domain of human p53

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Title
Local structural elements in the mostly unstructured transcriptional activation domain of human p53
Author(s)
Hyun Lee; K Hun Mok; Ranjith Muhandiram; Kyu Hwan Park; Jae Eun Suk; Do Hyung Kim; Jun Chang; Young Chul Sung; Kwan Yong Choi; Kyou Hoon Han
Bibliographic Citation
Journal of Biological Chemistry, vol. 275, no. 38, pp. 29426-29432
Publication Year
2000
Abstract
DNA transcription is initiated by a small regulatory region of transactivators known as the transactivation domain. In contrast to the rapid progress made on the functional aspect of this promiscuous domain, its structural feature is still poorly characterized. Here, our multidimensional NMR study reveals that an unbound full-length p53 transactivation domain, although similar to the recently discovered group of loosely folded proteins in that it does not have tertiary structure, is nevertheless populated by an amphipathic helix and two nascent turns. The helix is formed by residues Thr18-Leu26 (Thr-Phe-Ser-Asp-Leu-Trp-Lys-Leu-Leu), whereas the two turns are formed by residues Met40-Met44 and Asp48-Trp53, respectively. It is remarkable that these local secondary structures are selectively formed by functionally critical and positionally conserved hydrophobic residues present in several acidic transactivation domains. This observation suggests that such local structures are general features of acidic transactivation domains and may represent 'specificity determinants' (Ptashne, M., and Gann, A. A. F. (1997), Nature 386, 569-577) that are important for transcriptional activity.
ISSN
0021-9258
Publisher
Amer Soc Biochemistry Molecular Biology Inc
DOI
http://dx.doi.org/10.1074/jbc.M003107200
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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