Effect of short interspersed element sequences on the integration and expression of a reporter gene in the preimplantation-stage mouse embryos

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Title
Effect of short interspersed element sequences on the integration and expression of a reporter gene in the preimplantation-stage mouse embryos
Author(s)
Yong Kook KangJung Sun Park; Chul Sang Lee; Young Il Yeom; Yong Mahn Han; An Sik Chung; Kyung Kwang Lee
Bibliographic Citation
Molecular Reproduction and Development, vol. 56, no. 3, pp. 366-371
Publication Year
2000
Abstract
Based on the assumption that foreign DNA sequences may have increased chance of integration into the host genome if they are flanked by high copy- numbered genomic sequences such as SINEs (short interspersed elements), we investigated the integration frequency of Lac Z reporter gene flanked by a fused B1/B2 in an in vivo system using pronuclear microinjection technique in the mouse. The SINE-flanked DNA showed a 4-fold increased integration frequency of the reporter gene than the control DNA (63% vs. 16%). Moreover, the level of β-galactosidase expression, estimated from the X-Gal staining intensity in transgenic embryos, was greatly higher in SINE-carrying DNA. These results suggest that the SINE sequences can serve a very useful tool in improving the efficiency of current transgenic animal technology.
Keyword
transgeneSINEintegrationmicroinjection
ISSN
1040-452X
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/1098-2795(200007)56:3<366::AID-MRD6>3.0.CO;2-O
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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