Application of fibrin zymography for determining the optimum culture time for protease activity

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Title
Application of fibrin zymography for determining the optimum culture time for protease activity
Author(s)
Nack Shick Choi; Seung Ho Kim
Bibliographic Citation
Biotechnology Techniques, vol. 13, pp. 899-901
Publication Year
1999
Abstract
The culture time for maximal enzyme production by bacteria was determined by SDS-fibrin zymography. Bacillus subtilis and B. amyloliquefaciens secreted several extracellular proteases during the cellular lysis after 40 h and 30 h, respectively. Using this technique, we determined which protein possessed activity and would be isolated before the process of purification began. This proposed method offers an effective harvesting time in which to obtain the proteins having maximal activity.
Keyword
Bacillusfibrinproteasezymography
ISSN
0951-208X
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1008942611827
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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