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- Title
- Genomic organization and tissue-specific expression of rat urocortin
- Author(s)
- Jung Hyun Park; Yun Jung Lee; Shin Young Na; Kil Lyong Kim
- Bibliographic Citation
- Neuroscience Letters, vol. 292, no. 1, pp. 45-48
- Publication Year
- 2000
- Abstract
- The genomic organization of rat urocortin was determined using both cDNA and liver genomic DNA as templates for polymerase chain reactions. A single intron of 261 bp was found upstream to the ATG start codon, and the whole urocortin coding sequence was shown to be contained in a single exon. Relying on these results, oligonucleotide primers were designed, which can differentiate genomic DNA contamination from cDNA-derived signals in a reverse transcription-polymerase chain reaction. Tissue screening for urocortin expression revealed that the mid-brain is the major site of urocortin mRNA expression, and that other peripheral organs, including lymphoid tissues and peripheral blood lymphocytes, do not produce urocortin.
- Keyword
- raturocortingenomic organizationintronperipheral blood lymphocytesreverse transcription-polymerase chain reaction
- ISSN
- 0304-3940
- Publisher
- Elsevier
- DOI
- http://dx.doi.org/10.1016/S0304-3940(00)01434-8
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
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