Genomic organization and tissue-specific expression of rat urocortin

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Title
Genomic organization and tissue-specific expression of rat urocortin
Author(s)
Jung Hyun Park; Yun Jung Lee; Shin Young Na; Kil Lyong Kim
Bibliographic Citation
Neuroscience Letters, vol. 292, no. 1, pp. 45-48
Publication Year
2000
Abstract
The genomic organization of rat urocortin was determined using both cDNA and liver genomic DNA as templates for polymerase chain reactions. A single intron of 261 bp was found upstream to the ATG start codon, and the whole urocortin coding sequence was shown to be contained in a single exon. Relying on these results, oligonucleotide primers were designed, which can differentiate genomic DNA contamination from cDNA-derived signals in a reverse transcription-polymerase chain reaction. Tissue screening for urocortin expression revealed that the mid-brain is the major site of urocortin mRNA expression, and that other peripheral organs, including lymphoid tissues and peripheral blood lymphocytes, do not produce urocortin.
Keyword
raturocortingenomic organizationintronperipheral blood lymphocytesreverse transcription-polymerase chain reaction
ISSN
0304-3940
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0304-3940(00)01434-8
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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