Involvement of SH2-SH2-SH3 domain of phospholipase cγ1 in NF-κB signaling

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Title
Involvement of SH2-SH2-SH3 domain of phospholipase cγ1 in NF-κB signaling
Author(s)
Bo Yeon Kim; Dae Ook Kang; Won Keun Oh; Jin Hee Kim; Yong Kyung Choe; Jong Soo Jang; Pann Gill Suh; Sung Ho Ryu; Tae Ick MheenJong Seog Ahn
Bibliographic Citation
FEBS Letters, vol. 472, no. 1, pp. 45-49
Publication Year
2000
Abstract
To directly define the role of phospholipase Cγ1 (PLCγ1) in NF-κB activation, NF-κB promoted luciferase reporter gene plasmid (pNF-κB-Luc) was transfected into rat-3Y1 fibroblasts that overexpress whole PLCγ1 (PLCγ1-3Y1), src homology domains SH2-SH2-SH3 of PLCγ1 (SH223-3Y1) and v-src (Src-3Y1). Transient transfection with pNF-κB-Luc remarkably increased the luciferase activity in all three transformants compared with normal rat-3Y1 cells. Pretreatment with inhibitors of protein tyrosine kinase reduced this increase in luciferase activity, but U73122 (a PLC inhibitor) did not. While PD98059, an inhibitor of mitogen activated protein kinase (MAPK), significantly reduced the luciferase activity, there was no effect by wortmannin and Ro-31-8220, inhibitors of phosphatidylinositol 3-kinase (PI3K) and protein kinase C (PKC), respectively. This study shows a direct evidence that the SH2-SH2-SH3 region of PLCγ1 contributes to the NF-κB signaling and that MAPK, but not PI3K and PKC, is involved in SH2-SH2-SH3 mediated NF-κB activation in these cells.
Keyword
Mitogen activated protein kinaseNuclear factor κBPhosphatidylinositol 3-kinasePhospholipase Cγ1Protein kinase CSH2-SH3
ISSN
0014-5793
Publisher
Wiley
DOI
http://dx.doi.org/10.1016/S0014-5793(00)01415-0
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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