Detection of cellular receptors specific for the hepatitis B virus preS surface protein on cell lines of extrahepatic origin

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Title
Detection of cellular receptors specific for the hepatitis B virus preS surface protein on cell lines of extrahepatic origin
Author(s)
Jung Hyun Park; Eun A Choi; Eun Wie Cho; Yun Jung Lee; Jung Min Park; Shin Young Na; Kil Lyong Kim
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 277, no. 1, pp. 246-254
Publication Year
2000
Abstract
Hepatitis B virus infection is primarily mediated by the interaction of the preS region of the viral envelope protein with its still unknown cellular receptor. Using recombinantly expressed preS proteins, the distribution of preS-binding receptors on cell lines from extra-hepatic origins was determined by immunofluorescence and flow cytometry. In contrast to human liver cell lines, most cell lines from extrahepatic origins did not bind preS proteins. Nevertheless, exceptions were found in the bone marrow, derived cell line, KG-1, and the osteogenic sarcoma cell line SaOS-2, as well as in the previously reported EBV-transformed B-cell line, Wa. To determine the biochemical nature of these receptors, Wa-cells were cell surface biotinylated and the preS-binding receptors were isolated by immunoprecipitation. A specific band with a molecular weight of -39 kDa was identified in a SDS-polyacrylamide gel, which further characterization is expected to provide clues regarding the infection mechanism of HBV in hepatic- and extra-hepatic cells.
Keyword
hepatitis B viruspreS proteinextrahepatic cellsreceptorflow cytometry
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1006/bbrc.2000.3661
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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