Synthesis of recombinant human interleukin-2 via controlled feed of lactose-complex media in fed-batch cultures of Escherichia coli BL21(DE3)[pT7-G3IL2]

Cited 7 time in scopus
Metadata Downloads
Title
Synthesis of recombinant human interleukin-2 via controlled feed of lactose-complex media in fed-batch cultures of Escherichia coli BL21(DE3)[pT7-G3IL2]
Author(s)
Vibhor Saraswat; Jee Won Lee; Dae Young Kim; Young Hoon Park
Bibliographic Citation
Biotechnology Letters, vol. 22, no. 4, pp. 261-265
Publication Year
2000
Abstract
Using lactose as an inducer, recombinant human interleukin-2 (rhIL-2) was synthesized with an N-terminus fusion partner, G3 (three tandem-arranged glucagon peptides) in fed-batch cultures at high cell concentration (60-90 g l-1) of Escherichia coli BL21(DE3) [pT7-G3IL2]. With batch additions of lactose (4 x 13.5 g), the fusion rhIL-2 was synthesized up to 9.3 g l-1. However, if all the lactose (54 g) was added at once to the culture, synthesized fusion rhIL-2 decreased to 5.4 g l-1 with a decreased cell growth rate. A statistical optimization of the production medium containing glucose, yeast extract, and lactose led to fusion rhIL-2 being produced at > 9 g l-1.
Keyword
Escherichia coliInterleukin-2lactosesymport
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005638602146
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.