High level expression of human lipocortin(annexin) 1 in Escherichia coli

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Title
High level expression of human lipocortin(annexin) 1 in Escherichia coli
Author(s)
Kwang Lae Hoe; Eun Hee Cho; Soo Ok Lee; Doe Sun Na
Bibliographic Citation
Biotechnology Letters, vol. 18, no. 2, pp. 163-168
Publication Year
1996
Abstract
Human lipocortin (annexin) 1, a member of the annexin family of phospholipid binding proteins, has previously been expressed in E. coli (Huh et al., 1990). To improve the expression level of lipocortin 1 in E. coli, several expression vectors containing either the PL or the Ptrc promoter were constructed. The highest expression level, up to 20% of the total E. coli proteins, was obtained with plasmid pHT3. Plasmid pHT3 contains the pUC origin, the lipocortin 1 cDNA under Ptrc promoter, and the locI gene.
Keyword
lipocortin 1escherichia coliexpression vector
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00128673
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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