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- Title
- High level expression of human lipocortin(annexin) 1 in Escherichia coli
- Author(s)
- Kwang Lae Hoe; Eun Hee Cho; Soo Ok Lee; Doe Sun Na
- Bibliographic Citation
- Biotechnology Letters, vol. 18, no. 2, pp. 163-168
- Publication Year
- 1996
- Abstract
- Human lipocortin (annexin) 1, a member of the annexin family of phospholipid binding proteins, has previously been expressed in E. coli (Huh et al., 1990). To improve the expression level of lipocortin 1 in E. coli, several expression vectors containing either the PL or the Ptrc promoter were constructed. The highest expression level, up to 20% of the total E. coli proteins, was obtained with plasmid pHT3. Plasmid pHT3 contains the pUC origin, the lipocortin 1 cDNA under Ptrc promoter, and the locI gene.
- Keyword
- lipocortin 1escherichia coliexpression vector
- ISSN
- 0141-5492
- Publisher
- Springer
- Full Text Link
- http://dx.doi.org/10.1007/BF00128673
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
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