Isolation and purification of methyl mercaptan oxidase from thiobacillus thiooxidans for detection of mercaptans = 머갑탄류 검출을 위한 Thiobacillus thiooxidans가 생산하는 메칠머갑탄 산화효소의 분리 및 정제

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Title
Isolation and purification of methyl mercaptan oxidase from thiobacillus thiooxidans for detection of mercaptans = 머갑탄류 검출을 위한 Thiobacillus thiooxidans가 생산하는 메칠머갑탄 산화효소의 분리 및 정제
Author(s)
Sang Joon Kim; Hyun Jae Shin; Dae Sil Lee; Ji Won Yang
Bibliographic Citation
Korean Journal of Biotechnology and Bioengineering, vol. 15, no. 2, pp. 145-149
Publication Year
2000
Abstract
Methyl mercaptan oxidase was isolated and purified from Thiobacillus thiooxidans KCTC2505 for the detection of mercaptans. The procedure of purification involved DEAE-Sephacel and Superose 12 column chromatographies with recovery yields of 40 and 6.3%, and specific activity of 19.7 and 80.1 units/mg-protein, respectively. The molecular weight of purified methyl mercaptan oxidase was determined to be 68.1 kDa by SDS-PAGE. The extract from DEAE-Sephacel column chromatography had a high activity in oxidizing methyl mercaptan to produce formaldehyde which can be easily detected by purpald-coloring method. Optimum temperature for activity was observed at 43 degrees. This enzyme was activated by NH4CI and (NH4)2HO4, and inhibited by KCI and NaCI.
Keyword
methyl mercaptan oxidasethiobacillus thiooxidanspurpaldenzyme purification
ISSN
1225-7117
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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