Effect of cross-linking agents on L-sorbose production by immobilized Gluconobacter suboxydans cells

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dc.contributor.authorYoung Min Park-
dc.contributor.authorSang Ki Rhee-
dc.contributor.authorEui Sung Choi-
dc.contributor.authorIn Sik Chung-
dc.date.accessioned2017-04-19T08:57:45Z-
dc.date.available2017-04-19T08:57:45Z-
dc.date.issued1998-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5376-
dc.description.abstractBiological oxidation of D-sorbitol to L-sorbose using permeated and immobilized cells of Gluconobacter suboxydans was carded out to investigate the optimum reaction condition. The stabilization effect of cross-linking agents such as glutaraldehyde, tannic acid, and polyethyleneimine to prevent the leakage of enzymes from beads containing permeated and immobilized cells of G. suboxydans was examined by the production of L-sorbose from the mixture of D-sorbitol and gluconic acid. The protein concentration effused from immobilized beads treated with only glutaraldehyde was 5.2 μg/ml after 20 h. The beads of G. suboxydans immobilized with alginate and cross-linked with 0.3% glutaraldehyde was the most useful for the oxidation of D-sorbitol to L- sorbose.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleEffect of cross-linking agents on L-sorbose production by immobilized Gluconobacter suboxydans cells-
dc.title.alternativeEffect of cross-linking agents on L-sorbose production by immobilized Gluconobacter suboxydans cells-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number6-
dc.citation.endPage699-
dc.citation.startPage696-
dc.citation.volume8-
dc.contributor.affiliatedAuthorSang Ki Rhee-
dc.contributor.affiliatedAuthorEui Sung Choi-
dc.contributor.alternativeName박영민-
dc.contributor.alternativeName이상기-
dc.contributor.alternativeName최의성-
dc.contributor.alternativeName정인식-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 8, no. 6, pp. 696-699-
dc.subject.keywordcross-linking agents-
dc.subject.keywordD-sorbitol-
dc.subject.keywordglutaraldehyde-
dc.subject.keywordL- sorbose-
dc.subject.keywordpolyethyleneimine-
dc.subject.localcross-linking agents-
dc.subject.localD-sorbitol-
dc.subject.localGlutaraldehyde-
dc.subject.localglutaraldehyde-
dc.subject.localL- sorbose-
dc.subject.localpolyethyleneimine-
dc.description.journalClassY-
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Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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