Effect of cross-linking agents on L-sorbose production by immobilized Gluconobacter suboxydans cells

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Title
Effect of cross-linking agents on L-sorbose production by immobilized Gluconobacter suboxydans cells
Author(s)
Young Min Park; Sang Ki Rhee; Eui Sung Choi; In Sik Chung
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 8, no. 6, pp. 696-699
Publication Year
1998
Abstract
Biological oxidation of D-sorbitol to L-sorbose using permeated and immobilized cells of Gluconobacter suboxydans was carded out to investigate the optimum reaction condition. The stabilization effect of cross-linking agents such as glutaraldehyde, tannic acid, and polyethyleneimine to prevent the leakage of enzymes from beads containing permeated and immobilized cells of G. suboxydans was examined by the production of L-sorbose from the mixture of D-sorbitol and gluconic acid. The protein concentration effused from immobilized beads treated with only glutaraldehyde was 5.2 μg/ml after 20 h. The beads of G. suboxydans immobilized with alginate and cross-linked with 0.3% glutaraldehyde was the most useful for the oxidation of D-sorbitol to L- sorbose.
Keyword
cross-linking agentsD-sorbitolglutaraldehydeL- sorbosepolyethyleneimine
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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