Differential inheritance modes of DNA methylation between euchromatic and heterochromatic DNA sequences in ageing fetal bovine fibroblasts

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Title
Differential inheritance modes of DNA methylation between euchromatic and heterochromatic DNA sequences in ageing fetal bovine fibroblasts
Author(s)
Yong Kook Kang; Deog Bon Koo; Jung Sun Park; Young Hee Choi; Kyung Kwang Lee; Yong Mahn Han
Bibliographic Citation
FEBS Letters, vol. 498, no. 1, pp. 1-5
Publication Year
2001
Abstract
To elucidate overall changes in DNA methylation occurring by inappropriate epigenetic control during ageing, we compared fetal bovine fibroblasts and their aged neomycin-resistant versions using bisulfite-PCR technology. Reduction in DNA methylation was observed in euchromatic repeats (18S-rRNA/art2) and promoter regions of single-copy genes (the cytokeratin/β-lactoglobulin/interleukin-13 genes). Contrastingly, a stable maintenance of DNA methylation was revealed in various heterochromatic sequences (satellite I/II/alphoid and Bov-B). The differential inheritance mode of DNA methylation was confirmed through the analysis of individual neomycin-resistant clones. These global, multi-locus analyses provide evidence on the tendency of differential epigenetic modification between genomic DNA regions during ageing.
Keyword
ageingmethylationepigenetic modificationeuchromatinheterochromatin
ISSN
0014-5793
Publisher
Wiley
DOI
http://dx.doi.org/10.1016/S0014-5793(01)02472-3
Type
Article
Appears in Collections:
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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