Secretory expression of human growth hormone in Saccharomyces cerevisiae using three different leader sequences

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Title
Secretory expression of human growth hormone in Saccharomyces cerevisiae using three different leader sequences
Author(s)
Moon Sun Hahm; Bong Hyun Chung
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 6, no. 4, pp. 306-309
Publication Year
2001
Abstract
A recombinant human growth hormone (hGH) was expressed as a secretory product in the yeast Saccharomyces cerevisiae. Three different leader sequences derived from the mating factor α1 (MFα1), inulinase and invertase were used to direct the secretion of hGH into the extracellular medium. Among three leader sequences tested, the inulinase leader sequence was found to be the most efficient in the secretory expression of hGH. In contrast, no hGH was detected in the extracellular medium with the invertase leader sequence. After 48 h shake-flask culture, the yields of hGH secreted into the medium by the invertase, MFα1, inulinase and invertase leader sequences were approximately 0, 0.3 and 0.9 mg/L, respectively. The secretion efficiencies were also found to be 0, 3.8 and 13% for the invertase, MFα1 and inulinase leader sequences, respectively.
Keyword
human growth hormonesecretionleader sequenceSaccharomyces cerevisiae
ISSN
1226-8372
Publisher
South Korea
DOI
http://dx.doi.org/10.1007/BF02931995
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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