Hepatitis B virus X 형질전환 생쥐와 인체 간세포암종에서 proliferating cell nuclear antigen 발현과 자멸사

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Title
Hepatitis B virus X 형질전환 생쥐와 인체 간세포암종에서 proliferating cell nuclear antigen 발현과 자멸사
Author(s)
Hyung Bae Moon; Dae Yeul Yu; Hyung Ryun Yoo; Byung Joon So; Kwon Mook Chae; Hak Cheol Kim; Ki Jung Yun; Won Cheol Han; Hyang Jeong Jo; Bo Yong Kim
Bibliographic Citation
Korean Journal of Pathology, vol. 35, no. 2, pp. 129-136
Publication Year
2001
Abstract
Background : This experiment was designed to study the cell kinetics of hepatocellular carcinoma (HCC) in both hepatitis B virus X (HBx) transgenic mice and humans. Methods : The immunohistochemical stain of proliferating cell nuclear antigen (PCNA) and TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay of apoptosis were used on formalin fixed-paraffin embedded tissues. Results : PCNA labeling indices (PCNA-LI) in the liver of HBx transgenic mice were markedly increased in HCC (11.3%) compare to the dysplastic areas (1.3%) and in the liver of non-transgenic littermates (0.1%). There was no significant difference of PCNA-LI in the dysplastic areas between HCC developed mice and non-HCC developed mice. Apoptosis labeling indices (Apoptosis-LI) in both the dysplastic areas and HCC of HBx transgenic mice were similar to those of non-transgenic littermates. PCNA-LI was markedly increased in human HCC (28.9%) compare to the background of HCC (2.9%) and the control liver (2.9%). Apoptosis-LI was decreased in human HCC (0.3%) compare to the background of HCC (0.4%) and the control liver (1.0%). Conclusion : There is a marked increase of cell proliferating activity in human HCC and in HCC of HBx transgenic mice, and there is a decrease of apoptosis in human HCC, but not in HCC of HBx transgenic mice.
Keyword
transgenichepatocellularcarcinomaproliferating cell nuclear antigenapoptosis
ISSN
1738-1843
Publisher
South Korea
Type
Article
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1. Journal Articles > Journal Articles
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