Production of herbicide-resistant transgenic plants from embryogenic suspension cultures of cucumber = 오이의 배발생 현탁 배양세포로부터 제초제 저항성 형질전환 식물체 생산

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Title
Production of herbicide-resistant transgenic plants from embryogenic suspension cultures of cucumber = 오이의 배발생 현탁 배양세포로부터 제초제 저항성 형질전환 식물체 생산
Author(s)
Je Wook Woo; Won Joong Chung; Kwan Sam Choi; Hyo Guen Park; Nam Kwon Baek; Jang Ryol Liu
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 28, no. 1, pp. 53-58
Publication Year
2001
Abstract
To develop herbicide-resistant cucumber plants (Cucumis sativus L. cv Green Angle) embryogenic suspension cultures were co-cultured with Agrobacterium tumefaciens strain LBA4404 carrying a disarmed binary vector pGA-bar. The T-DNA region of this binary vector contains the nopalin synthase/neomycin phosphotransferase Ⅱ (npt Ⅱ) chimeric gene for kanamycin resistance and the cauliflower 35S/phosphinothricin acetyltransferase (bar) chimeric gene for phosphinothricin (PPT) resistance, After co-cultivation for 48 h, embryogenic calli were placed on maturation media containing 20 mg/L PPT. Approximately 200 putatively transgenic plantlets were obtained in hormone free media containing 40 mg/L PPT. Northern blot hybridization analysis confirmed the expression of the bar gene that was integrated into the genome of five transgenic plants. Transgenic cucumber plants were grown to maturity. Mature plants in soil showed tolerance to the commercial herbicide (Basta) of PPT at the manufacturer's suggested level (3 mL/L).
Keyword
agrobacterium tumefacienscucumis sativus L.herbicide-resistancesomatic embryogenesistransformation
ISSN
1015-5880
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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