Typical demethylation events in cloned pig embryos : clues on specis-specific differnces in epigenetic reprogramming of a cloned donor genome

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Title
Typical demethylation events in cloned pig embryos : clues on specis-specific differnces in epigenetic reprogramming of a cloned donor genome
Author(s)
Yong Kook Kang; Deog Bon Koo; Jung Sun Park; Yong Hee Choi; Ha Na Kim; Won Kyong Chang; Kyung Kwang Lee; Yong Mahn Han
Bibliographic Citation
Journal of Biological Chemistry, vol. 276, no. 43, pp. 39980-39984
Publication Year
2001
Abstract
We investigated whether a genomic demethylation process occurs in pig preimplantation embryos produced by either normal fertilization or somatic cell nuclear transfer. The methylation status of the centromeric satellite and the PRE-1 short interspersed element (SINE) sequences was characterized using bisulfite-sequencing technology. Typical demethylation processes were identified in these repetitive sequences of the cloned donor genome during cleavage, the patterns of which were similar to the ones detected in fertilized counterparts. These findings are different from previous observations with cloned bovine embryos where various repeated regions of the donor genome exhibited aberrant methylation patterns. Our results indicate that species-specific differences exist in modifying the epigenetic status of cloned donor genomes.
ISSN
0021-9258
Publisher
Amer Soc Biochemistry Molecular Biology Inc
DOI
http://dx.doi.org/10.1074/jbc.M106516200
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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