Effect of DNA conformation on genomic integration of transgenes and its implications on integration mechanism = 외래유전자의 게놈내 삽입에 있어서 DNA 형태가 미치는 영향

Cited 0 time in scopus
Metadata Downloads
Title
Effect of DNA conformation on genomic integration of transgenes and its implications on integration mechanism = 외래유전자의 게놈내 삽입에 있어서 DNA 형태가 미치는 영향
Author(s)
Yong Kook KangJung Sun Park; Chul Sang Lee; Yong Mahn Han; Kyung Kwang Lee
Bibliographic Citation
Reproductive & Developmental Biology, vol. 25, no. 3, pp. 237-242
Publication Year
2001
Abstract
We recently demonstrated that both linear- and supercoil-form B1/B2 SINE (short interspersed elements) sequenced could increase an intergration frequency of a reporter gene in preimplatation mouse embroys. In those reports, when either a control or SINE-flanked DNA was separately applied to microinjection, the proportions of β-gal positive were 16% and 63%, respectively, in liner-formDNA, and 6% and 25%, respectively, in circular-form DNA. Here, we examined the contribution of a circular-form DNA moiety to integration frequency by using a mixed-form (linear and circular-form) DNA in microinjection. When examined in the blastocyst stage, the proportion of β-gal-positive embroyos was 17.3% and 46.6% in control and SINE-flanked DNA, respectively. These results suggest that there is little contribution of circular-form DNA moiety to the resultant intergration frequency, and that the majority of the integration events are mediated through a linear confornation of vector DNA. In addition, some clues on integration process could be obtained from the analysis of microinjection results.
ISSN
I000-0006
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.