Cloning and characterization of the Mycobacterium bovis BCG panB gene encoding ketopantoate hydroxylmethyltransferase

Cited 0 time in scopus
Metadata Downloads
Title
Cloning and characterization of the Mycobacterium bovis BCG panB gene encoding ketopantoate hydroxylmethyltransferase
Author(s)
Jin Koo Kim; Kwang Dong Kim; Jong-Seok Lim; Hee Gu Lee; Sang Jae Kim; Sang Hyun Cho; Won Hwa Jeong; In Seong Choe; Tai Wha Chung; Sang Gi Paik; Yong Kyung Choe
Bibliographic Citation
BMB Reports, vol. 34, no. 4, pp. 342-346
Publication Year
2001
Abstract
The Mycobacterium bovis BCG panB gene, encoding ketopantoate hydroxymethyltransferase (KPHMT), was cloned from a λgt11 genomic library and sequenced. The DNA sequence encodes a protein that contains 281 amino acid residues (Mr 29,337) with a high similarity to the KPHMTs. Subcloning of a 846 bp open reading frame (ORF), but not a 735 bp ORF, into the vector pUC19 led to complementation of the panB mutant of Escherichia coli. The BCG panB gene was overexpressed in E. coli and the KPHMT purified to homogeneity. The recombinant protein was further confirmed by an enzymatic assay.
Keyword
BCGKetopantoate hydroxymethyltransferase (KPHMT)panBPantothenic acid
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.