Open Access@KRIBBDivision of A.I. & Biomedical ResearchImmunotherapy Research Center1. Journal Articles
Cloning and characterization of the Mycobacterium bovis BCG panB gene encoding ketopantoate hydroxylmethyltransferase
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- Title
- Cloning and characterization of the Mycobacterium bovis BCG panB gene encoding ketopantoate hydroxylmethyltransferase
- Author(s)
- Jin Koo Kim; Kwang Dong Kim; Jong-Seok Lim; Hee Gu Lee; Sang Jae Kim; Sang Hyun Cho; Won Hwa Jeong; In Seong Choe; Tai Wha Chung; Sang Gi Paik; Yong Kyung Choe
- Bibliographic Citation
- BMB Reports, vol. 34, no. 4, pp. 342-346
- Publication Year
- 2001
- Abstract
- The Mycobacterium bovis BCG panB gene, encoding ketopantoate hydroxymethyltransferase (KPHMT), was cloned from a λgt11 genomic library and sequenced. The DNA sequence encodes a protein that contains 281 amino acid residues (Mr 29,337) with a high similarity to the KPHMTs. Subcloning of a 846 bp open reading frame (ORF), but not a 735 bp ORF, into the vector pUC19 led to complementation of the panB mutant of Escherichia coli. The BCG panB gene was overexpressed in E. coli and the KPHMT purified to homogeneity. The recombinant protein was further confirmed by an enzymatic assay.
- ISSN
- 1225-8687
- Publisher
- Korea Soc-Assoc-Inst
- Type
- Article
- Appears in Collections:
- Division of A.I. & Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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