Identification of total extracellular fibrinase from Bacillus sp. DJ using one- or two- dimensional fibrin Zymography for proteomic approach

Cited 0 time in scopus
Metadata Downloads
Title
Identification of total extracellular fibrinase from Bacillus sp. DJ using one- or two- dimensional fibrin Zymography for proteomic approach
Author(s)
Nack Shick Choi; Jin Young Lee; Kab Seog Yoon; Kyoung Yoen Han; Seung Ho Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 11, no. 6, pp. 1111-1114
Publication Year
2001
Abstract
An extracellular fibrinolytic-enzyme-producing bacterium was isolated from Doen-Jang, a Korean traditional fermented food, and identified as Bacillus sp. DJ based on its morphology and cellular fatty acid composition. The total extracellular fibrinase (EF) from Bacillus sp. DJ was analyzed using three fibrin zymographic techniques, SDS-fibrin zymography (SDS-FZ), isoelectrofocucing-fibrin zymography (IEF-FZ), and a two-dimensional SDS-fibrin zymographic analysis (2D SDS-FZ). As a result, the EF map of Bacillus sp. DJ was established. The results suggest that the 2D SDS-FZ method will be a useful tool for the proteomic approach for many other bacterial proteases.
Keyword
bacillusfibrinaseproteomicstwo-dimensional fibrin zymography
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.