A caspase inducing inhibitor isolated from Forsythiae fructus = 연교(Forsythiae fructus)로부터 분리한 caspase 유도 저해물질

Cited 0 time in scopus
Metadata Downloads
Title
A caspase inducing inhibitor isolated from Forsythiae fructus = 연교(Forsythiae fructus)로부터 분리한 caspase 유도 저해물질
Author(s)
Jin Hee Kim; Yung Hee Kho; Mee Ree Kim; Hyun A Kim; Sang Myung Lee; Choong Hwan Lee
Bibliographic Citation
Korean Journal of Food Science & Technology, vol. 34, no. 1, pp. 114-117
Publication Year
2002
Abstract
During the screening of inhibitors of caspase-3 induction in U937 human monocytic leukemia cells from natural sources, Forsythiae fructus, which showed a high level of inhibition, was selected. And then, the compound was purified from the methanol extract using silica gel column chromatography and HPLC. The inhibitor was identified as rengyolone, by spectroscophic methods of ESI-MS, , , DEPT, and HMBC. Rengyolone showed inhibitory activity of caspase-3 induction, a major protease of apoptosis cascade, with an value of after 7 h of treatment in U937 cells. It also showed inhibitory activity of caspace-1 induction, with an value of after 40 h of treatment in D10S cells. In addition, it showed protective effect against cell death with an value of on U937 cells induced by etoposide after 24 h of treatment, but did not show any cytotoxicity at the same condition without etoposide, a caspase 3 inducing agent.
Keyword
apoptosisrengyoloneetoposideU937D10S
ISSN
0367-6293
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.