Relationship between acrylamide concentration and enzymatic activity in an improved single fibrin zymogram gel system

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Title
Relationship between acrylamide concentration and enzymatic activity in an improved single fibrin zymogram gel system
Author(s)
Nack Shick Choi; Byoung Young Kim; Jin Young Lee; Kab Seog Yoon; Kyoung Yoen Han; Seung Ho Kim
Bibliographic Citation
BMB Reports, vol. 35, no. 2, pp. 236-238
Publication Year
2002
Abstract
Based on the zymography analysis, Bacillus sp. DJ-4 (screened from Doen-Jang, a Korean traditional fermented food) secretes seven extracellular fibrinolytic enzymes (EFEs; 68, 64, 55, 45, 33, 27, and 13 kDa) in culture broth. These seven EFEs were analyzed by newly applied SDS-fibrin zymography combined with gradient polyacrylamide (SDS-FZGP). This improved gel system was used with a 5-20% acrylamide gradient in a fibrin zymogram gel for the separation of proteins with molecular masses from below 10 kDa to over 100 kDa on one gel plate. Using this system, high molecular weight bands (HMWBs) were clearly and sharply resolved. We also examined the relationship between an acrylamide concentration and the enzymatic activity of EFE using densitometric analysis.
Keyword
bacillusextracellular proteasefibrin zymography
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5483/bmbrep.2002.35.2.236
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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