Novel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-α

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Title
Novel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-α
Author(s)
Ha Ryoung PooJae Jun Song; Seung Pyo Hong; Yoon Ho Choi; Sun Woo Yoon; Jin Ho Kim; Sang Chul LeeSeung Goo Lee; Moon Hee Sung
Bibliographic Citation
Biotechnology Letters, vol. 24, no. 14, pp. 1185-1189
Publication Year
2002
Abstract
A new E. coli expression system, the pHCE-IIB vector, using a constitutively expressing HCE promoter derived upstream from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toebii, was developed for the high-level expression of foreign proteins without induction. The expression efficiency of recombinant human tumor necrosis factor-α (rhTNFα) using pHCE-IIB was compared with that of the pET14B vector under the control of a T7 promoter induced by IPTG. The total amount of rhTNFα produced by the pHCE system was approximately twice that produced by the pET system under optimal fermentation conditions thereby demonstrating the convenience and economical advantage of the new constitutive pHCE-IIB expression vector over the conventional pET expression vector.
Keyword
Constitutive expressionIsopropyl-beta-D-thiogalactosidasePHCERecombinant human tumor necrosis factor
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1016107230825
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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