Introduction of VP6 gene into potato plant by Agrobacterium-mediated transformation and analysis of VP6 expression in transgenic potatoes = Rotavirus VP6 유전자의 감자식물체내로의 도입과 형질전환체의 발현분석

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Title
Introduction of VP6 gene into potato plant by Agrobacterium-mediated transformation and analysis of VP6 expression in transgenic potatoes = Rotavirus VP6 유전자의 감자식물체내로의 도입과 형질전환체의 발현분석
Author(s)
Jung Won Youm; Jae Heung Jeon; Jae Yeol Jung; Byoung Chan Lee; Won Jin Kang; Mi Sun Kim; Chul Joong Kim; Hyouk Joung; Hyun Soon Kim
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 29, no. 2, pp. 93-98
Publication Year
2002
Abstract
A VP6 fragments was subcloned with BamHI in the binary pMBP-1 vector under Califlower Mosaic Virus (CaMV) 355 promoter and neomycin phosphotransferase II (npt II) gene. The recombinant binary vector was mobilized into Agrobacterium-tumefaciens LBA4404 by the freeze-thaw method and potato (Solanum tubensum L. cv Desiree) was transformed by modified leaf-disc cocultivation. Shoots were induced on MS medium with 0.01 mg/L NAA, 0.1 mg/L GA_3, 2.0 mg/L Zeatin, 100.0 mg/L kanamycin, 500.0 mg/L carbenicillin. In order to identify the copy number of VP6 into potato plant, total genomic DNA was isolated from transgenic potato and analysed by Southern blotting. Genomic DNA and total mRNA analysis demonstrated the incorporation of the foreign gene into the potato genome, as well as their transcription.
Keyword
solanum tuberosum L. cv DesireeVP6 geneCaMV 35S promoterNPT Ⅱ
ISSN
1015-5880
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2002.29.2.093
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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