DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hyun Kyu Park | - |
dc.contributor.author | Y S Huh | - |
dc.contributor.author | Jee Won Lee | - |
dc.contributor.author | I H Kim | - |
dc.date.accessioned | 2017-04-19T08:59:25Z | - |
dc.date.available | 2017-04-19T08:59:25Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1225-7117 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5970 | - |
dc.description.abstract | Ferritins are heteropolymers composed of H- and L-chains with a variable ratio. The H-chain ferritin is better for iron uptake, oxidation and physical stability than L-chain ferritin. Therefore, in this study the expression of H-chain ferritin in recombinant E. coli at 37 led to the accumulation of recombinant ferritin. An insoluble form of ferritin was separated from disrupted cells. followed by various primary separation steps with two kinds of butters. Collected samples from the steps were purilied by DEAE-cellulose gels packed in a column. The fractions from the DEAE column were assayed to obtain the amount and the purity of ferritin by using RP- and GF-HPLC. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Purification of heavy chain ferritin(Fh) from recombinant E. coli = 재조합 E. coli로부터 철단백질의 정제 | - |
dc.title.alternative | Purification of heavy chain ferritin(Fh) from recombinant E. coli | - |
dc.type | Article | - |
dc.citation.title | Korean Journal of Biotechnology and Bioengineering | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 451 | - |
dc.citation.startPage | 446 | - |
dc.citation.volume | 17 | - |
dc.contributor.affiliatedAuthor | Hyun Kyu Park | - |
dc.contributor.affiliatedAuthor | Jee Won Lee | - |
dc.contributor.alternativeName | 박현규 | - |
dc.contributor.alternativeName | 허윤석 | - |
dc.contributor.alternativeName | 이지원 | - |
dc.contributor.alternativeName | 김인호 | - |
dc.identifier.bibliographicCitation | Korean Journal of Biotechnology and Bioengineering, vol. 17, no. 5, pp. 446-451 | - |
dc.identifier.doi | 10.1142/9789812702623_0135 | - |
dc.subject.keyword | ferritin | - |
dc.subject.keyword | purification | - |
dc.subject.keyword | E.coli | - |
dc.subject.keyword | ion exchange chromatography(IEC) | - |
dc.subject.keyword | HPLC | - |
dc.subject.local | ferritin | - |
dc.subject.local | Ferritin | - |
dc.subject.local | Purification | - |
dc.subject.local | purification | - |
dc.subject.local | Purifcation | - |
dc.subject.local | Escherichia coli. | - |
dc.subject.local | escherichia coli | - |
dc.subject.local | Escherichia Coli | - |
dc.subject.local | Escherichia coli | - |
dc.subject.local | E.coli | - |
dc.subject.local | escherichia coil | - |
dc.subject.local | E. coli | - |
dc.subject.local | E. Coli | - |
dc.subject.local | ion exchange chromatography(IEC) | - |
dc.subject.local | HPLC | - |
dc.description.journalClass | N | - |
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