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- Title
- Purification of heavy chain ferritin(Fh) from recombinant E. coli = 재조합 E. coli로부터 철단백질의 정제
- Author(s)
- Hyun Kyu Park; Y S Huh; Jee Won Lee; I H Kim
- Bibliographic Citation
- Korean Journal of Biotechnology and Bioengineering, vol. 17, no. 5, pp. 446-451
- Publication Year
- 2002
- Abstract
- Ferritins are heteropolymers composed of H- and L-chains with a variable ratio. The H-chain ferritin is better for iron uptake, oxidation and physical stability than L-chain ferritin. Therefore, in this study the expression of H-chain ferritin in recombinant E. coli at 37 led to the accumulation of recombinant ferritin. An insoluble form of ferritin was separated from disrupted cells. followed by various primary separation steps with two kinds of butters. Collected samples from the steps were purilied by DEAE-cellulose gels packed in a column. The fractions from the DEAE column were assayed to obtain the amount and the purity of ferritin by using RP- and GF-HPLC.
- Keyword
- ferritinpurificationion exchange chromatography(IEC)HPLCE.coli
- ISSN
- 1225-7117
- Publisher
- Korea Soc-Assoc-Inst
- DOI
- http://dx.doi.org/10.1142/9789812702623_0135
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
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