Purification of heavy chain ferritin(Fh) from recombinant E. coli = 재조합 E. coli로부터 철단백질의 정제

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Title
Purification of heavy chain ferritin(Fh) from recombinant E. coli = 재조합 E. coli로부터 철단백질의 정제
Author(s)
Hyun Kyu Park; Y S Huh; Jee Won Lee; I H Kim
Bibliographic Citation
Korean Journal of Biotechnology and Bioengineering, vol. 17, no. 5, pp. 446-451
Publication Year
2002
Abstract
Ferritins are heteropolymers composed of H- and L-chains with a variable ratio. The H-chain ferritin is better for iron uptake, oxidation and physical stability than L-chain ferritin. Therefore, in this study the expression of H-chain ferritin in recombinant E. coli at 37 led to the accumulation of recombinant ferritin. An insoluble form of ferritin was separated from disrupted cells. followed by various primary separation steps with two kinds of butters. Collected samples from the steps were purilied by DEAE-cellulose gels packed in a column. The fractions from the DEAE column were assayed to obtain the amount and the purity of ferritin by using RP- and GF-HPLC.
Keyword
ferritinpurificationion exchange chromatography(IEC)HPLCE.coli
ISSN
1225-7117
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1142/9789812702623_0135
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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