GSH2, a gene encoding γ-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha
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- Title
- GSH2, a gene encoding γ-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha
- Author(s)
- V M Ubiyvovk; T Y Nazarko; O G Stasyk; Min Jeong Sohn; Hyun Ah Kang; A A Sibirny
- Bibliographic Citation
- FEMS Yeast Research, vol. 2, no. 3, pp. 327-332
- Publication Year
- 2002
- Abstract
- The GSH2 gene, encoding Hansenula polymorpha γ-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3-kb XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the Candida albicans homologue (Gcs1p). In comparison to the Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The GSH2 sequence appears in the GenBank database under accession No. AF435121.
- Keyword
- γ-Glutamylcysteine synthetaseGlutathione biosynthesisHansenula polymorphaMethylotrophic yeast
- ISSN
- 1567-1356
- Publisher
- Oxford Univ Press
- DOI
- http://dx.doi.org/10.1016/S1567-1356(02)00111-3
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
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