GSH2, a gene encoding γ-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha

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Title
GSH2, a gene encoding γ-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha
Author(s)
V M Ubiyvovk; T Y Nazarko; O G Stasyk; Min Jeong Sohn; Hyun Ah Kang; A A Sibirny
Bibliographic Citation
FEMS Yeast Research, vol. 2, no. 3, pp. 327-332
Publication Year
2002
Abstract
The GSH2 gene, encoding Hansenula polymorpha γ-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3-kb XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the Candida albicans homologue (Gcs1p). In comparison to the Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The GSH2 sequence appears in the GenBank database under accession No. AF435121.
Keyword
γ-Glutamylcysteine synthetaseGlutathione biosynthesisHansenula polymorphaMethylotrophic yeast
ISSN
1567-1356
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1016/S1567-1356(02)00111-3
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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