Screening of monocyte chemoattractant protein-1-induced chemotaxis inhibitors from medicinal herbs = 생약자원으로부터 monocyte chemoattractant protein-1에 의한 chemotaxis 저해활성 검색

Abstract

Blood monocytes are the precursors for the lipid-laden foam cells of early atherosclerotic lesions. Monocyte chemoattractant protein-1 (MCP-1), a CC chemokine, and chemokine receptor 2 (CCR2) play a crucial role in the recruitment of monocytes to the vascular lesion. Using the human monocyte THP-1 cell line, we investigate the inhibitory effects of methanol extracts of 127 medicinal herbs on MCP-1-induced chemotaxis. Seven kinds of methanol extracts of medicinal herbs showed above 40% inhibitory effect with the concentration of 25 μg/ml. They were divide three fractions of CHCl3, BuOH, H2O to use solvent partition. Among them, butanol extract of Junci Medulla and CHCl3 extract of Clematidis Radix are showed significant inhibitory activities (above 50% inhibition) at the same concentration.