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- Title
- Low-dose gamma irradiation as means of isolating carotenoid-hyperproducing yeast mutant
- Author(s)
- N K Sun; S H Lee; G H Ahn; Mi Sun Won; K B Song
- Bibliographic Citation
- Journal of Microbiology and Biotechnology, vol. 12, no. 6, pp. 1010-1012
- Publication Year
- 2002
- Abstract
- In order to isolate carotenoid-hyperproducing yeast, low-dose gamma irradiation was used as means of mutagenesis. Phaffia rhodozyma was treated by gamma irradiation of less than 10 kGy, which is considered to be a wholesome irradiation condition established by the Food and Drug Administration. Through repeated rounds of gamma irradiation and visual screening, mutant 3A4-8 was obtained. It produced a 3,824 μg carotenoid/g yeast, 69% higher content than 2,265 μg/g yeast of the unirradiated one. This result indicates that low-dose gamma irradiation could be used as means of mutagenesis to obtain carotenoid-hyperproducing strain of Phaffia rhodozyma, since only carotenoid-hyperproducing yeast survived gamma irradiation by scavenging oxygen radicals generated by radiolysis of water.
- Keyword
- carotenoidmutantγ-irradiation
- ISSN
- 1017-7825
- Publisher
- Korea Soc-Assoc-Inst
- Type
- Article
- Appears in Collections:
- Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
- Files in This Item:
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