Low-dose gamma irradiation as means of isolating carotenoid-hyperproducing yeast mutant

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Title
Low-dose gamma irradiation as means of isolating carotenoid-hyperproducing yeast mutant
Author(s)
N K Sun; S H Lee; G H Ahn; Mi Sun Won; K B Song
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 12, no. 6, pp. 1010-1012
Publication Year
2002
Abstract
In order to isolate carotenoid-hyperproducing yeast, low-dose gamma irradiation was used as means of mutagenesis. Phaffia rhodozyma was treated by gamma irradiation of less than 10 kGy, which is considered to be a wholesome irradiation condition established by the Food and Drug Administration. Through repeated rounds of gamma irradiation and visual screening, mutant 3A4-8 was obtained. It produced a 3,824 μg carotenoid/g yeast, 69% higher content than 2,265 μg/g yeast of the unirradiated one. This result indicates that low-dose gamma irradiation could be used as means of mutagenesis to obtain carotenoid-hyperproducing strain of Phaffia rhodozyma, since only carotenoid-hyperproducing yeast survived gamma irradiation by scavenging oxygen radicals generated by radiolysis of water.
Keyword
γ-irradiationcarotenoidmutant
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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