Characterization of extracellular α-galactosidase produced by Streptomyces sp. YB-4 = 균체외 α-galactosidase를 생산하는 Streptomyces sp. YB-4의 분리 및 효소 특성

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Title
Characterization of extracellular α-galactosidase produced by Streptomyces sp. YB-4 = 균체외 α-galactosidase를 생산하는 Streptomyces sp. YB-4의 분리 및 효소 특성
Author(s)
S Y Kim; K H Cho; Chang Jin KimDong Jin Park; K H Yoon
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 30, no. 4, pp. 332-338
Publication Year
2002
Abstract
A strain YB-4 producing the extracellular α-galactosidase was isolated from soil, and has been identified as Streptomyces sp. on the basis of its cultural, morphological and physiological properties. The partially purified α-galactosidase was most active on paranitrophenyl-α -D-galactopyranoside at pH 6.0 and 60°C. The enzyme retained 90% of its maximum activity between pH 4.0 and pH 10.0 after pre-incubation for 1 h. The enzyme was able to hydrolyze oligomeric substrates such as melibiose, raffinose and stachyose to liberate galactose residue, indicating that the α-galactosidase of Steptomyces sp. YB-4 hydrolyzed α-1,6 linkage.
Keyword
α-galactosidaseidentificationpropertyreaction productstreptomyces
ISSN
0257-2389
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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