Functional over-expression of the Stm1 protein, a G-protein-coupled receptor, in Schizosaccharomyces pombe

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Title
Functional over-expression of the Stm1 protein, a G-protein-coupled receptor, in Schizosaccharomyces pombe
Author(s)
Kyung Sook Chung; Dong Uk Kim; Sung Woo Ryoo; Eun Jung Kang; Mi Sun Won; Li La Kim; Young Joo Jang; P J Maeng; S C Kim; Hyang Sook Yoo; Kwang Lae Hoe
Bibliographic Citation
Biotechnology Letters, vol. 25, no. 3, pp. 267-272
Publication Year
2003
Abstract
We report here the first functional over-expression of the Stm1 protein, a G-protein-coupled receptor with seven-trans-membrane spanning regions, in a homologous expression system without internal modification of the open reading frame of Stm1. The entire coding sequence, except for the termination codon followed by a C-terminal His6 tag, has been cloned into the pREP1 vector. The functionally active Stm1-His6 was over-expressed in Schizosaccharomyces pombe under the control of the nmt1 (no message in thiamine) promoter. The expression after induction was 120 times as much as that of control before induction and it gave ∼500 ng protein/2 × 107cells.
Keyword
G-protein-coupled receptorHis6 tagHomologous expressionSchizosaccharomyces pombeSeven transmembrane protein
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1022355102192
Type
Article
Appears in Collections:
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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