Molecular characterization of NbPAF encoding the α6 subunit of the 20S proteasome in Nicotiana benthamiana

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Title
Molecular characterization of NbPAF encoding the α6 subunit of the 20S proteasome in Nicotiana benthamiana
Author(s)
Moonil Kim; Kyoung Sil Yang; Yu Kyung Kim; K H Paek; Hyun Sook Pai
Bibliographic Citation
Molecules and Cells, vol. 15, no. 1, pp. 127-132
Publication Year
2003
Abstract
The 26S proteasome involved in degradation of proteins covalently modified with polyubiquitin consists of the 20S proteasome and 19S regulatory complex. The NbPAF gene encoding the α6 subunit of the 20S proteasome was identified from Nicotiana benthamiana. NbPAF exhibits high sequence homology with the corresponding genes from Arabidopsis, human and yeast. The deduced amino acid sequence of NbPAF reveals that this protein contains the proteasome α-type subunits signature and nuclear localization signal at the N-terminus. The genomic Southern blot analysis suggests that the N. benthamiana genome contains one copy of NbPAF. The NbPAF mRNA was detected abundantly in flowers and weakly in roots and stems, but it was almost undetectable in mature leaves. In response to stresses, accumulation of the NbPAF mRNA was stimulated by methyl jasmonate, NaCl and salicylic acid, but not by abscisic acid and cold treatment in leaves. The NbPAF-GFP fusion protein was localized in the cytoplasm and nucleus.
Keyword
inducible expressionplant defense responsesingle genesubcellular localization
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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