Dykellic acid inhibits drug-induced caspase-3-like protease activation

Cited 7 time in scopus
Metadata Downloads
Title
Dykellic acid inhibits drug-induced caspase-3-like protease activation
Author(s)
Sang-Han Lee; Eun Soo Youk; Ho Jae Lee; Yung Hee Kho; Hwan Mook Kim; Sung Uk Kim
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 302, no. 3, pp. 539-544
Publication Year
2003
Abstract
Dykellic acid is a novel microbial metabolite isolated from the broth of Westerdykella multispora F50733. Investigations on the molecular function of dykellic acid revealed that this compound partially inhibits calcium influx, resulting in a decrease in Ca2+-dependent endonuclease activation and DNA fragmentation induced by camptothecin. In our experiments, active caspase-3-like protease cleavage of procaspase-3, PARP, and cytosolic cytochrome c was inhibited by dykellic acid in a concentration-dependent manner when the apoptosis was induced by camptothecin as well as doxorubicin. We confirmed that dykellic acid did not bind to camptothecin using surface plasmon resonance analysis. These results suggest that dykellic acid inhibits drug-induced apoptosis via a caspase-3-like protease-suppressing mechanism. Our data provide important information on the mechanism of action of dykellic acid and indicate that this compound may be employed in the treatment of specific caspase-3-like protease-mediated diseases.
Keyword
Apoptosis inhibitorCamptothecinCaspase-3-like proteaseDykellic acidInhibitor
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0006-291X(03)00210-9
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.