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- Title
- Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding
- Author(s)
- Srinivas Ramisetti; Hyun Ah Kang; Sang Ki Rhee; Chul Ho Kim
- Bibliographic Citation
- Biotechnology and Bioprocess Engineering, vol. 8, no. 3, pp. 183-186
- Publication Year
- 2003
- Abstract
- The artificial gene coding for anticoagulant hirudin was placed under the control of the GAL 10 promoter and expressed in the galactokinase-deficient strain (Δgal1) of Saccharomyces cerevisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L.
- Keyword
- fed-batch fermentationGAL promotergalactoserecombinant hirudinsaccharomyces cerevisiae
- ISSN
- 1226-8372
- Publisher
- Springer
- DOI
- http://dx.doi.org/10.1007/BF02935894
- Type
- Article
- Appears in Collections:
- Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
- Files in This Item:
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