Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding

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Title
Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding
Author(s)
Srinivas Ramisetti; Hyun Ah Kang; Sang Ki Rhee; Chul Ho Kim
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 8, no. 3, pp. 183-186
Publication Year
2003
Abstract
The artificial gene coding for anticoagulant hirudin was placed under the control of the GAL 10 promoter and expressed in the galactokinase-deficient strain (Δgal1) of Saccharomyces cerevisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L.
Keyword
fed-batch fermentationGAL promotergalactoserecombinant hirudinsaccharomyces cerevisiae
ISSN
1226-8372
Publisher
South Korea
DOI
http://dx.doi.org/10.1007/BF02935894
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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