Enhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene

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Title
Enhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene
Author(s)
Suk Yoon Kwon; Sun Mee Choi; Young Ock Ahn; Heang Soon Lee; H B Lee; Y M Park; Sang Soo Kwak
Bibliographic Citation
Journal of Plant Physiology, vol. 160, no. 4, pp. 347-353
Publication Year
2003
Abstract
To analyze the physiological role of dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzing the reduction of DHA to ascorbate in environmental stress adaptation, T1 transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants expressing a human DHAR gene in chloroplasts were biochemically characterized and tested for responses to various stresses. Fully expanded leaves of transgenic plants had about 2.29 times higher DHAR activity (units/g fresh wt) than non-transgenic (NT) plants. Interestingly, transgenic plants also showed a 1.43 times higher glutathione reductase activity than NT plants. As a result, the ratio of AsA/DHA was changed from 0.21 to 0.48, even though total ascorbate content was not significantly changed. When tobacco leaf discs were subjected to methyl viologen (MV) at 5 μmol/L and hydrogen peroxide (H2O2) at 200 mmol/L, transgenic plants showed about a 40% and 25% reduction in membrane damage relative to NT plants, respectively. Furthermore, transgenic seedlings showed enhanced tolerance to low temperature (15°C) and NaCl (100 mmol/L) compared to NT plants. These results suggest that a human derived DHAR properly works for the protection against oxidative stress in plants.
Keyword
Ascorbic acidDehydroascorbate reductaseGlutathioneMethyl viologenOxidative stressTransgenic tobacco plant
ISSN
0176-1617
Publisher
Elsevier
DOI
http://dx.doi.org/10.1078/0176-1617-00926
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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