High frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis
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- Title
- High frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis
- Author(s)
- Suk Weon Kim; D S In; Tae Jin Kim; Jang Ryol Liu
- Bibliographic Citation
- Plant Cell Tissue and Organ Culture, vol. 74, no. 2, pp. 163-167
- Publication Year
- 2003
- Abstract
- Culture conditions are described for high frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis Max. Petiole explants formed embryogenic calluses at a frequency of 53% when cultured on B5 medium supplemented with 13.6 μM 2,4-dichlorophenoxyacetic acid (2,4-D) alone. Leaf explants formed embryogenic calluses at a frequency of 21% when cultured at a combination of 4.52 μM 2,4-D and 2.22 μM 6-benzyladenine. Cell suspension cultures were established with petiole-derived embryogenic calluses using liquid B5 medium with 4.52 μM 2,4-D. Upon plating onto B5 basal medium, cell suspension cultures produced numerous somatic embryos, which then developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse.
- Keyword
- Somatic embryoMedicinal plantPapaveracea
- ISSN
- 0167-6857
- Publisher
- Springer
- DOI
- http://dx.doi.org/10.1023/A:1023997627578
- Type
- Article
- Appears in Collections:
- Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
- Files in This Item:
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