Separation and purification of teicoplanin by diaion HP-20 and concanavalin A chromatography = 소수성 diaion HP-20 및 친화성 concanavalin A 크로마토그래피를 이용한 glycopeptide계 항생제 teicoplanin의 분리 및 정제

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Title
Separation and purification of teicoplanin by diaion HP-20 and concanavalin A chromatography = 소수성 diaion HP-20 및 친화성 concanavalin A 크로마토그래피를 이용한 glycopeptide계 항생제 teicoplanin의 분리 및 정제
Author(s)
Jae-Chan Lee; Hae Ryong Park; Dong Jin park; Y B Kim; Chang-Jin Kim
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 31, no. 2, pp. 201-204
Publication Year
2003
Abstract
Glycopeptide antibiotics, teicoplanin was purified from a mutant strain of Actinoplanes teichomyceticus ATCC31121, A. teichomyceticus MSL2211. We developed a simple procedure to separate and purify the teicoplanin from the fermentation broth. Teicoplanin was purified by two-step purification system, hydrophobic adsorption and sugar affinity chromatography in combination with HPLC analysis based on the properties of hydrophobic acyl chain and sugar moiety in teicoplanin. Teicoplanin was separated from the culture broth by Diaion HP-20 and further purified by concanavalin A affinity column chromatography. As an adsorbent resin, Diaion HP-20 in broth eliminated toxic effects on growth, reduced feedback repression of teicoplanin production, and assisted in rapid recovery of teicoplanin. The teicoplanin displayed the final yield of 80% and 95% of purity.
Keyword
actinoplanes teicomyceticusconcanavalin Adiaion HP-20teicoplanin
ISSN
0257-2389
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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