Suppression of RelA/p65 transactivation activity by a lignoid manassantin isolated from Saururus chinensis

Cited 28 time in scopus
Metadata Downloads
Suppression of RelA/p65 transactivation activity by a lignoid manassantin isolated from Saururus chinensis
Jeong-Hyung Lee; B Y Hwang; Kyung Sook Kim; Jeong Beom Nam; Young-Soo Hong; Jung Joon Lee
Bibliographic Citation
Biochemical Pharmacology, vol. 66, no. 10, pp. 1925-1933
Publication Year
In our search for NF-κB inhibitors from natural resources, we have previously identified two structurally related dilignans, manassantin A and B as specific inhibitors of NF-κB activation from Saururus chinensis. However, their molecular mechanism of action remains unclear. We here demonstrate that manassantins A and B are potent inhibitors of NF-κB activation by the suppression of transciptional activity of RelA/p65 subunit of NF-κB. These compounds significantly inhibited the induced expression of NF-κB reporter gene by LPS or TNF-α in a dose-dependent manner. However, these compounds did not prevent the DNA-binding activity of NF-κB assessed by electrophoretic mobility shift assay as well as the induced-degradation of IκB-α protein by LPS or TNF-α. Further analysis revealed that manassantins A and B dose-dependently suppressed not only the induced NF-κB activation by overexpression of RelA/p65, but also transactivation activity of RelA/p65. Furthermore, treatment of cells with these compounds prevented the TNF-α-induced expression of anti-apoptotic NF-κB target genes Bfl-1/A1, a prosurvival Bcl-2 homologue, and resulted in sensitizing HT-1080 cells to TNF-α-induced cell death. Similarly, these compounds also suppressed the LPS-induced inducible nitric oxide synthase expression and nitric oxide production. Taken together, manassantins A and B could be valuable candidate for the intervention of NF-κB-dependent pathological condition such as inflammation and cancer.
apoptosislignoidsNF-κBRelA/p65saururus chinensistransactivation
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.