Scale-up of recombinant hirudin production from Saccharomyces cerevisiae

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Title
Scale-up of recombinant hirudin production from Saccharomyces cerevisiae
Author(s)
Chul Ho Kim; K J Rao; D J Youn; Sang Ki Rhee
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 8, no. 5, pp. 303-305
Publication Year
2003
Abstract
Scale-up of hirudin production from Saccharomyces cerevisiae from bench-scale to pilot-scale was carried out based on constant volumetric oxygen transfer coefficient (KLa). Fed-batch mode of cultivation using step-wise feeding strategy of galactose was employed for the production of hirudin in a 30-L and a 300-L pilot-scale fermentor. The final hirudin concentrations were achieved 390 mg/L and 286.1 mg/L, and the volumetric productivities were 80.4% and 90.7% with the 30-L and 300-L fermentors, respectively, compared to the productivity of the 5-L bench-scale fermentor.
Keyword
fed batch fermentationGAL promoterrecombinant hirudinsaccharomyces cerevisiaescale-up
ISSN
1226-8372
Publisher
South Korea
DOI
http://dx.doi.org/10.1007/BF02949222
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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