Anticarcinogenic effect of Kimchi extract on 2-AAF-induced rat hepatocarcinogenesis system = 김치추출물의 2-AAF로 유도된 쥐 간발암과정 억제효과

Abstract

Anticarcinogenic effect of the methanol extract from kimchi was determined in a new medium-term carcinogenicity bioassay using D-galactosamine (DGA) as a nonsurgical method to induce liver cell regeneration in place of partial hepatectomy (PH). Male F344 rats were initially given a single i.p. injection of diethylnitrosamine (DEN, 200 mg/kg) and received 2 i.p. injections of D-galactosamine (DGA, 300 mg/kg) at the end of week 2 and 5. They were treated with oral administration of 2-acetylaminofluorene (2-AAF, 7 mg/kg) and kimchi extract (920 mg/kg) for week 3～8 (5 days/week). Anticarcinogenic effect was assessed by comparing the numbers and areas per square centimeter of glutathione S-transferase placental form-positive (GST-P+) foci in the livers of kimchi extract-treated animals with those of the control animals given 2-AFF alone. No difference was noted in body weights between control and kimchi treated group. Liver weights and liver/body weight ratios were not changed in rats treated with kimchi sample. The mean numbers of GST-P+ foci in livers of control and kimchi-treated group were 13.8 and 8.8/cm2, respectively. The areas of GST-P+ foci were decreased in kimchi extract-administrated group. These results suggest that kimchi has a inhibitory effect on 2-AAF-induced hepatocarcinogenesis.