Sequence-based approach to finding functional lipases from microbial genome databases

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Title
Sequence-based approach to finding functional lipases from microbial genome databases
Author(s)
H K Kim; Yeo Jin Jung; Won Chan Choi; Hyoung Seok Ryu; Tae Kwang Oh; Jung-Kee Lee
Bibliographic Citation
FEMS Microbiology Letters, vol. 235, no. 2, pp. 349-355
Publication Year
2004
Abstract
A sequence-based approach was used to retrieve functional lipases from microbial genome databases. Many novel genes assigned as putative lipases were tested using the criteria of the typical lipase sequence rule, based on a consensus sequence of a catalytic triad (Ser, Asp, His) and oxyanion hole sequence (HG). To obtain the lipase genes satisfying the sequence rule, PCR cloning was performed, while the lipase activities were tested using a tributyrin/tricaprylin plate and p-nitrophenyl caproate. Among nine putative lipases from four strains, five functional lipolytic proteins were obtained from Archaeoglobus fulgidus, Deinococcus radiodurans, and Agrobacterium tumefaciens. All five lipases exhibited a relatively low sequence similarity (less than 26.7%) with known lipases and turned out to belong to different lipase families. Accordingly, the current results indicate that the proposed strategic approach based on the microbial genome is an efficient and rapid method for finding novel and functional lipases.
Keyword
agrobacterium tumefaciensarchaeoglobus fulgidusdeinococcus radioduranslipasemicrobial genome database
ISSN
0378-1097
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1016/j.femsle.2004.05.004
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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