Human hepatitis B virus-X protein alters mitochondrial function and physiology in human liver cells

Cited 132 time in scopus
Metadata Downloads
Title
Human hepatitis B virus-X protein alters mitochondrial function and physiology in human liver cells
Author(s)
Young Ik Lee; Jung Me Hwang; Jee Hye Im; Yoon Ik Lee; Nam-Soon Kim; D G Kim; Dae Yeul Yu; H B Moon; S K Park
Bibliographic Citation
Journal of Biological Chemistry, vol. 279, no. 15, pp. 15460-15471
Publication Year
2004
Abstract
The hepatitis B virus-X protein (HBx) regulates fundamental aspects of mitochondrial physiology. We show that HBx down-regulates mitochondrial enzymes involved in electron transport in oxidative phosphorylation (complexes I, III, IV, and V) and sensitizes the mitochondrial membrane potential in a hepatoma cell line. HBx also increases the level of mitochondrial reactive oxygen species and lipid peroxide production. HBx does not activate apoptotic signaling, although it sensitizes hepatoma cells to apoptotic signaling, which is dependent on reactive oxygen species. Increased intrahepatic lipid peroxidation in HBx transgenic mice demonstrated that oxidative injury occurs as a direct result of HBx expression. Therefore, we conclude that mitochondrial dysfunction is a crucial pathophysiological factor in HBx-expressing hepatoma cells and provides an experimental rationale in the investigation of mitochondrial function in rapidly renewed tissues, as in hepatocellular carcinomas.
ISSN
0021-9258
Publisher
Amer Soc Biochemistry Molecular Biology Inc
DOI
http://dx.doi.org/10.1074/jbc.M309280200
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.