Identification of a cytochrome P450 hydroxylase gene involved in rifamycin biosynthesis by Amycolatopsis mediterranei S699

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Title
Identification of a cytochrome P450 hydroxylase gene involved in rifamycin biosynthesis by Amycolatopsis mediterranei S699
Author(s)
S K Lee; C Y Choi; Jong Seog Ahn; J Y Cho; C S Park; Y J Yoon
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 14, no. 2, pp. 356-362
Publication Year
2004
Abstract
In analyzing the region of the Amycolatopsis mediterranei S699 chromosome responsible for the biosynthesis of the ansamycin antibiotic rifamycin, we identified a gene, designated orf0, which is located immediately upstream of the rifamycin polyketide synthase (PKS). Orf0 encodes a protein, on the basis of sequence-comparative analysis, that is similar to several cytochrome P450 monooxygenases from different sources. The rifamycin producer, A. mediterranei, predominantly produces rifamycin B from its macrocyclic intermediate, proansamycin X, through dehydrogenation and hydroxylation steps. However, an A. mediterranei strain, deleted in orf0 by gene replacement, no longer produced rifamycin B. Furthermore, a versatile replicative vector in A. mediterranei was constructed and rifamycin B production was restored in a complementation experiment of orf0 using this novel vector. These consecutive results verified that the Orf0 protein, which is a P450 hydroxylase, is required for the production of rifamycin B in A. mediterranei.
Keyword
amycolatopsis mediterraneiP450 monooxygenaseproansamycin Xreplication vectorrifamycin
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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